|Cell Line Name:||AtT20|
|Keywords:||Mouse LAF1 pituitary gland tumour|
|Cell Line Description:||Derived after alternate passage of LAF1 mouse pituitary tumour cells as tumours in animals and in cell culture. Cells produce adrenocorticotropic hormone (ACTH) at approximately the rate of 1000mU/mg of cell protein per week.|
|Morphology:||Small round cells growing as large aggregates|
|Growth Mode:||Aggregates in suspension|
|Subculture Routine:||This cell line loses viability when frozen and hence the expected viable cell count when resuscitated from frozen stock may be only 40 -50%. To sub-culture allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. A subcultivation ratio of 1:2 is recommended. Cells grow in large viable clusters making counting difficult. A seeding density of approximately 3 x 105/ml is recommended. 5% CO2; 37°C. Proliferation is enhanced by incubation on a shaking platform.|
|Culture Medium:||Ham's F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). Cells grow in large aggregates.|
|Products:||Adrenocorticotropic hormone (ACTH)|
|Depositor:||Obtained from ATCC|
|References:||FURTH J, GADSEN EL, UPTON AC 1953 ACTH secreting transplantable pituitary tumors. Proc Soc Exp Biol Med. 84(1):253-4 PMID: 13121002.|
|Additional Bibliography:||BUONASSISI V, SATO G, COHEN AI.1962 Hormone-producing cultures of adrenal and pituitary tumor origin. Proc Natl Acad Sci U S A. 48:1184-90 PMID: 13874682.|
|Patents:||None specified by Depositor|
|Research Council Deposit:||No|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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